RNA processing of beta-globin transcripts containing 5' flanking and structural gene sequences.
AUTOR(ES)
Winicov, I
RESUMO
RNA species have been identified in murine erythroid cells which contain both 5' flanking and structural gene sequences from the beta maj globin gene. Two nonpolyadenylated RNA transcripts, average 3700 and 1800-1900 nucleotides long, were identified by denaturing agarose gel electrophoresis and were found to hybridize to both 5' and 3' beta maj globin flanking sequences. This finding suggests that transcription initiated in the 5' flanking region does proceed past the polyadenylation site. The apparent higher concentration of the 5' flanking sequences in precursor RNA molecules, as compared to mature polyadenylated globin mRNA, suggests increased precursor stability of beta globin transcripts initiated in the 5' flanking region.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=334680Documentos Relacionados
- DNA sequences preceding the rabbit beta-globin gene are required for formation in mouse L cells of beta-globin RNA with the correct 5' terminus.
- Beta-globin gene promoter generates 5' truncated transcripts in the embryonic/fetal erythroid environment.
- Beta-globin gene promoter generates 5′ truncated transcripts in the embryonic/fetal erythroid environment
- Nuclear matrix DNA from chicken erythrocytes contains beta-globin gene sequences.
- The DNA sequence of the 5' flanking region of the human beta-globin gene: evolutionary conservation and polymorphic differences.