RNA Expression Profiles and Data Mining of Sugarcane Response to Low Temperature1

AUTOR(ES)

Nogueira, Fábio T.S.

FONTE

The American Society for Plant Biologists

RESUMO

Tropical and subtropical plants are generally sensitive to cold and can show appreciable variation in their response to cold stress when exposed to low positive temperatures. Using nylon filter arrays, we analyzed the expression profile of 1,536 expressed sequence tags (ESTs) of sugarcane (Saccharum sp. cv SP80-3280) exposed to cold for 3 to 48 h. Thirty-four cold-inducible ESTs were identified, of which 20 were novel cold-responsive genes that had not previously been reported as being cold inducible, including cellulose synthase, ABI3-interacting protein 2, a negative transcription regulator, phosphate transporter, and others, as well as several unknown genes. In addition, 25 ESTs were identified as being down-regulated during cold exposure. Using a database of cold-regulated proteins reported for other plants, we searched for homologs in the sugarcane EST project database (SUCEST), which contains 263,000 ESTs. Thirty-three homologous putative cold-regulated proteins were identified in the SUCEST database. On the basis of the expression profiles of the cold-inducible genes and the data-mining results, we propose a molecular model for the sugarcane response to low temperature.

Documentos Relacionados

• A Rice Membrane-Bound Calcium-Dependent Protein Kinase Is Activated in Response to Low Temperature1
• RESPIRATION AND CHEMICAL CHANGES OF THE PAPAYA FRUIT IN RELATION TO TEMPERATURE1
• Indole-3-Acetic Acid Metabolism in Lemna gibba Undergoes Dynamic Changes in Response to Growth Temperature1
• Aflatoxins: Production of the Toxins In Vitro in Relation to Temperature1
• NCBI GEO: mining millions of expression profiles—database and tools