Replicative intermediate of hepatitis B virus in transfected murine fibroblasts.
AUTOR(ES)
Zelent, A Z
RESUMO
The NIH 3T3-derived cell line psi AM22b, which carries a defective Moloney murine leukemia virus, was transfected with a plasmid carrying the neo gene and two head-to-tail copies of the hepatitis B virus (HBV) genome positioned with opposing polarities. Both the two HBV dimers and the neo gene were located between two Moloney murine leukemia virus long terminal repeats. Poly(A)+ RNAs isolated from one clone that grew in the presence of G418 contained the two major classes of HBV-specific transcripts (3.5-kilobase pregenome and 2.1-kilobase mRNAs) in approximately equivalent amounts, which was reminiscent of the profiles of viral mRNAs from the livers of infected humans and chimpanzees.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=255822Documentos Relacionados
- Expression of infectious woodchuck hepatitis virus in murine and avian fibroblasts.
- Induction of cell cycle progression by hepatitis B virus HBx gene expression in quiescent mouse fibroblasts.
- Telomere length predicts replicative capacity of human fibroblasts.
- Characterization of Rauscher murine leukemia virus envelope glycoprotein receptor in membranes from murine fibroblasts.
- In vitro production of immunosuppressive factors by murine sarcoma virus-transformed mouse fibroblasts.
