Regulated Expression of the Escherichia coli dam Gene

AUTOR(ES)

Calmann, Melissa A.

FONTE

American Society for Microbiology

RESUMO

Regulated expression of the Escherichia coli dam gene has been achieved with the araBAD promoter lacking a ribosome binding site. Cultures of dam mutants containing plasmid pMQ430 show no detectable methylation in the absence of arabinose and complete methylation in its presence. Dam methyltransferase is a substrate for the Lon protease.

Documentos Relacionados

• Role of SeqA and Dam in Escherichia coli gene expression: A global/microarray analysis
• The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene.
• SoxRS-Regulated Expression and Genetic Analysis of the yggX Gene of Escherichia coli
• Phenotypic reversal in dam mutants of Escherichia coli K-12 by a recombinant plasmid containing the dam+ gene.
• The DNA adenine methyltransferase (dam+) gene of bacteriophage T4 reverses the mutator phenotype of an Escherichia coli dam mutant.