Regulação do IRS-2 e da AKT em modelos animais de resistencia a insulina

AUTOR(ES)

Fernanda Alvarez Rojas

DATA DE PUBLICAÇÃO

2001

RESUMO

Insulin induced a wide variety of growth and metabolic responses in many cell types. These actions are initiated by insulin binding to its receptor, and involves a series of alternative and complementary p athways created by the multiple substrates of the insulin receptor (IRSs). In this study we investigated IRS-2 tyrosine phosphorylation, its association with PI 3-kinase and the phosphorylation of Akt, a serine/threonine kinase situated downstream to PI 3-kinase, in liver and muscle of 4 animal models of insulin resistance: the effect of acute epinephrine treatment (normoinsulinemia), the effect of chronic treatment with dexamethasone (hyperinsulinemia), 72 hours fasting and diabetes (STZ) (hipoinsulinemia). We used immunoprecipitation and immunoblotting techniques with specificantibodies: anti-IRS-2, anti-phsophotyrosine, anti-PI 3-kinase and anti-Akt. There was a downregulation in insulin-induced IRS-2 tyrosine phosphorylation and association with PI 3-kinase in liver and muscle of epinephrine and dexamethasone treated rats. Note: The complete abstract is available with the full electronic digital thesis or dissertations

ASSUNTO(S)

testes quimicos e reagentes insulina modelos animais em pesquisa

Documentos Relacionados

• Regulação da interação IRS-1/SHP2 em modelos de resistencia a insulina
• Novos substratos do receptor de insulina : regulação da proteina SHC em modelos animais de resistencia a insulina e do IRS-3 em celulas beta pancreaticas
• Regulação da proteina Janus Quinase 2 em modelos animais de resistencia a insulina
• Transmissão do sinal insulinico em coração e aorta : regulação em modelos animais de resistencia a insulinas
• Modulação da associação IR/PTP1B na transmissão do sinal da insulina em modelos animais de resistencia insulinica