Regulação da glicemia e secreção de insulina em camundongos transgenicos hipertrigliceridemicos

AUTOR(ES)
DATA DE PUBLICAÇÃO

2000

RESUMO

Glucose homeostasis and insulin secretion in transgenic mice overexpressing the human apolipoprotein CIII gene (apo cm tg) were studied. These mice have elevated plasma levels of triglycerides, ITee fatty acids (FF A) and cholesterol compared with control mice. The body weights, plasma glucose, and insulin levels, glucose disappearance rates and areas under the intraperitoneal Glucose Tolerance Test (ipGTT) curve for adult (4-8 month old) and aged (20-24 month old) apo cm tg mice were not different ITom those of control animals. However, an additional elevation of plasma FF A by treatment with heparin for 2-4 h impaired the ipGTT responses in transgenic mice compared to saline-treated mice (areas under the ipGTT curves; 18.7 :i: 1.0 mmol/L.120 min and 13.6 :i: 1.2 mmol/L.120 min for heparin- and saline-treated transgenic mice, respectively; n = 10 each; p <0.01). The glucose disappearance rate in heparin-treated transgenic mice (2.88 :i: 0.49 %/min; n=ll) was slightly lower than in heparin-treated controls (4.12 :i: 0.46 %/min; n =10 ; P <0.05). Glucose (22.2 mmol/L) stimulated insulin secretion in isolated islets to the same extent in saline-treated control and apo CIII tg mice. In islets ftom heparin-treated apo CIII tg mice, the insulin secretion at 2.8 and 22.2 mmol glucoseiL was lower than in heparin treated control mice. In conclusion, hypertriglyceridemia per se or a mild elevation in FF A did not affect insulin secretion or insulin resistance in adult or aged apo cm tg mice. Nonetheless, an additional elevation of FF A induced by heparin in hypertriglyceridemic mice impaired the ipGTT by reducing insulin secretion, and was probably associated with a marginal increase in insulin resistance

ASSUNTO(S)

camundongo insulina glicose acidos graxos

ACESSO AO ARTIGO

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