Recombinant mouse interferon-gamma regulation of antibody production.
AUTOR(ES)
Johnson, H M
RESUMO
Interferon-gamma produced in monkey cells by transfection with mouse interferon-gamma cDNA suppressed the mouse in vitro antibody response in a manner similar to that of natural mouse interferon-gamma. Significant suppression was obtained with as little as 1 U of interferon. Recombinant human interferon-gamma produced by cloning in a similar fashion was not suppressive. Both the suppressive and the antiviral activities of recombinant interferon-gamma were neutralized by antibodies to mouse natural interferon-gamma. Thus, interferon-gamma was responsible for the immunosuppression. At the cellular level, the recombinant interferon-gamma was capable of activating macrophages to suppress antibody production. The data provide clear-cut evidence that interferon-gamma plays an important role in regulation of immunological processes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=264676Documentos Relacionados
- Production of human interferon-gamma in serum-free medium.
- Interferon-gamma inhibits growth of Coxiella burnetii in mouse fibroblasts.
- Interferon-gamma inhibits macrophage apolipoprotein E production by posttranslational mechanisms.
- Requirement of endogenous interferon-gamma production for resolution of Listeria monocytogenes infection.
- Sequence of the porcine interferon-gamma (IFN-gamma) gene.