Real-Time TaqMan PCR for Quantifying Oral Bacteria during Biofilm Formation
AUTOR(ES)
Suzuki, Nao
FONTE
American Society for Microbiology
RESUMO
A TaqMan PCR was developed for quantifying early colonizer microorganisms in dental biofilms. To design species-specific primers and TaqMan probes, genomic subtractive hybridization was used. This quantitative assay in combination with subtractive hybridization may be of value in the study of microbial ecosystems consisting of related species that are involved in the formation and etiology of biofilms.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=497611Documentos Relacionados
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