Rastreabilidade de soja Roundup Ready® em produtos agrícolas e derivados: produção de materiais de referência e uso de marcadores AFLP / Roundup Ready® transgenic soybean traceability in agrifood products and derivatives: reference materials production and the use of AFLP markers
AUTOR(ES)
Othon Silva Abrahão
DATA DE PUBLICAÇÃO
2008
RESUMO
The introduction of soybean transgenic cultivars brought several changes in both world trade and regulation rules for producers and consumers, mainly in relation to certification, traceability, biosafety and labeling. Soybean is one of the largest commodities in the world trade and responsible for half of the Brazilian export incomings, present as raw material or ingredient in significant part of foods in the local market. The adoption of the threshold of 1,0 % for nonintentional presence of GMO in food, requiring obligatory labeling for contents above this level, demands reference materials for the control and quantification of transgenics in raw material and processed food, allowing the harmonization of results from different methods and laboratories, currently being produced exclusively by the Institute for Reference Materials and Measurements - IRMM, in Belgium. Due to the high costs and difficulty to obtain these products, candidate reference materials of Roundup Ready® transgenic soybean (RR soybean) were produced at levels of 0 %, 0,1 %, 0,5 %, 1,0 %, 2,0 % and 5,0 % of transgenic material, using cryogenic milling and mixing techniques, with subsequent verification by real time PCR. Tracing of Roundup Ready cultivars by origin was done through AFLP molecular markers in a total of 29 RR soybean cultivars: 21 Argentinean and 8 Brazilian cultivars. Fingerprinting was possible in 15 Argentinean and all Brazilian cultivars through 6 bands obtained from 4 selective pairs of primers, singled out from 67 different combinations of selective primers. For PCR analysis of processed food, primers were designed using freeware sources over the Internet (Primer3, NetPrimer, Rebase) for short fragments amplification (around 100 bp). Primers sets for the amplification of lectin and CP4 EPSPS gene fragments from RR soybean were designed for the detection of these genes in the 29 RR soybean cultivars and three soybean based food matrices, with the results validated by endonucleases digestion and sequencing
ASSUNTO(S)
food quality gmo quantification detecção de transgênicos rr soybean qualidade de alimentos transgenic detection soja rr quantificação de ogm
Documentos Relacionados
- Manejo de herbicidas na cultura da soja Roundup Ready®
- Utilização de chlorimuron-ethyl e imazethapyr na cultura da soja Roundup Ready®
- Associações entre o herbicida glyphosate e inseticidas na cultura da soja Roundup Ready®
- Detecção e quantificação de resíduos de soja Roundup Ready® em amostras de salsicha por PCR convencional e PCR em tempo real
- Controle de plantas voluntárias de soja Roundup Ready.