Rapid, reliable and inexpensive quality assessment of biotinylated cRNA
AUTOR(ES)
Zander, T., Yunes, J.A., Cardoso, A.A., Nadler, L.M.
FONTE
Brazilian Journal of Medical and Biological Research
DATA DE PUBLICAÇÃO
2006-05
RESUMO
The interpretation of oligonucleotide array experiments depends on the quality of the target cRNA used. cRNA target quality is assessed by quantitative analysis of the representation of 5' and 3' sequences of control genes using commercially available Test arrays. The Test array provides an economically priced means of determining the quality of labeled target prior to analysis on whole genome expression arrays. This manuscript validates the use of a duplex RT-PCR assay as a faster (6 h) and less expensive (
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