Rapid Method for the Radioisotopic Analysis of Gaseous End Products of Anaerobic Metabolism
AUTOR(ES)
Nelson, David R.
RESUMO
A gas chromatographic procedure for the simultaneous analysis of 14C-labeled and unlabeled metabolic gases from microbial methanogenic systems is described. H2, CH4, and CO2 were separated within 2.5 min on a Carbosieve B column and were detected by thermal conductivity. Detector effluents were channeled into a gas proportional counter for measurement of radioactivity. This method was more rapid, sensitive, and convenient than gas chromatography-liquid scintillation techniques. The gas chromatography-gas proportional counting procedure was used to characterize the microbial decomposition of organic matter in anaerobic lake sediments and to monitor 14CH4 formation from H2 and 14CO2 by Methanosarcina barkeri.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=186697Documentos Relacionados
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