Rapid method for direct extraction of DNA from soil and sediments.

AUTOR(ES)

Tsai, Y L

RESUMO

A rapid method for the direct extraction of DNA from soil and sediments was developed. The indigenous microorganisms in the soil and sediments were lysed by using lysozyme and a freeze-thaw procedure. The lysate was extracted with sodium dodecyl sulfate and phenol-chloroform. In addition to a high recovery efficiency (greater than 90%), the yields of DNA were high (38 and 12 micrograms/g [wet weight] from sediments and soil, respectively). This method generated minimal shearing of the extracted DNA. The crude DNA could be further purified with an Elutip-d column if necessary. An additional advantage of this method is that only 1 g of sample is required, which allows for the analysis of small samples and the processing of many samples in a relatively short (7 h) period.

Documentos Relacionados

• Comparison of methods of DNA extraction from stream sediments.
• Recovery of DNA from soils and sediments.
• Rapid Method for Direct Extraction of mRNA from Seeded Soils
• A rapid method for extraction and purification of DNA from dental plaque.
• Direct DNA extraction for PCR-mediated assays of soil organisms.