Rapid Identification of Yersinia enterocolitica in Blood by the 5′ Nuclease PCR Assay
AUTOR(ES)
Sen, Keya
FONTE
American Society for Microbiology
RESUMO
Yersinia enterocolitica accounts for 50% of the clinical sepsis episodes caused by the transfusion of contaminated red blood cells. A 5′ nuclease TaqMan PCR assay was developed to detect Y. enterocolitica in blood. Primers and a probe based on the nucleotide sequence of the 16S rRNA gene from Y. enterocolitica were designed. Whole-blood samples were spiked with various numbers of Y. enterocolitica cells, and total chromosomal DNA was extracted. When the TaqMan PCR assay was performed, as few as six bacteria spiked in 200 μl of blood could be detected. The assay was specific and did not detect other Yersinia species. The TaqMan assay is easy to perform, takes 2 h, and has the potential for use in the rapid detection of Y. enterocolitica contamination in stored blood units.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=86632Documentos Relacionados
- Rapid 5′ Nuclease (TaqMan) Assay for Detection of Virulent Strains of Yersinia enterocolitica†
- Validated 5′ Nuclease PCR Assay for Rapid Identification of the Genus Brucella
- 5′ Nuclease PCR Assay To Detect Yersinia pestis
- Development of a Fluorogenic 5′ Nuclease PCR Assay for Detection of the ail Gene of Pathogenic Yersinia enterocolitica
- Automated 5′ Nuclease PCR Assay for Identification of Salmonella enterica