Rapid Detection of Escherichia coli O157:H7 with Multiplex Real-Time PCR Assays
AUTOR(ES)
Jothikumar, Narayanan
FONTE
American Society for Microbiology
RESUMO
A SYBR Green LightCycler PCR assay using a single primer pair allowed simultaneous detection of stx1 and/or stx2 of Escherichia coli O157:H7. A distinct sequence of the Shiga-like toxin genes was amplified to yield products of 227 and/or 224 bp, respectively. The two products were distinguished by melting point curve analysis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=123919Documentos Relacionados
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