Rapid detection and identification of pathogenic mycobacteria by combining radiometric and nucleic acid probe methods.
AUTOR(ES)
Ellner, P D
RESUMO
The combination of radiometric methodology (BACTEC 12B) and probe technology for recovery and identification of mycobacteria was studied in two large hospital laboratories. The sediment from vials with positive growth indices was tested with DNA probes specific for Mycobacterium tuberculosis, Mycobacterium avium, and Mycobacterium intracellulare. The sensitivity of the radiometric method and the specificity of the probes resulted in a marked reduction in the time to the final report. Biochemical testing could be eliminated on isolates giving a positive reaction with one of the probes. Some 176 isolates of M. tuberculosis, 110 of M. avium, and 5 of M. intracellulare were recovered. Two-thirds of these isolates were detected and identified within 2 weeks of inoculation and the remainder was detected by 4 weeks, a reduction of 5 to 7 weeks to the final report.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=266607Documentos Relacionados
- Detection of yeast septicemia by biphasic and radiometric methods.
- Advances in nucleic acid-based detection methods.
- Detection of group D and viridans streptococci in blood by radiometric methods.
- Detection of urinary tract infections by rapid methods.
- Comparative evaluation of AccuProbe culture identification test for Neisseria gonorrhoeae and other rapid methods.