Rapid ATP assays in perfused mouse liver by 31P NMR.

AUTOR(ES)

McLaughlin, A C

RESUMO

31P NMR was used to continuously monitor ATP and inorganic phosphate levels in perfused mouse liver. Under "optimal" conditions, the time resolution of the technique was approximately 1 min. In the absence of any metabolic perturbations the ATP level remained constant for at least 2 hr and decreased by only approximately 20% in 18 hr. Both ATP and inorganic phosphate levels responded to alterations in the oxygen supply to the liver. The half-time for this response was approximately 1 min, and the response to short periods of hypoxia or ischemia was partially reversible. The addition of insulin caused only a minor decrease in the ATP level but significantly decreased the rate of response of ATP and phosphate levels to hypoxia and ischemia.

Documentos Relacionados

• Cerebral metabolic studies in vivo by 31P NMR.
• Quantitative studies of human cardiac metabolism by 31P rotating-frame NMR.
• Role of oxygen vs. glucose in energy metabolism in a mammary carcinoma perfused ex vivo: direct measurement by 31P NMR.
• A study of the conformation of 5S RNA by 31P NMR.
• Effects of ethanol on alanine metabolism in perfused mouse liver studied by 13C NMR.