Purification, crystallization and preliminary X-ray analysis of human mannose-binding lectin-associated serine protease-1 (MASP-1) catalytic region
AUTOR(ES)
Dobó, József
FONTE
International Union of Crystallography
RESUMO
Acidic nondenaturing PAGE revealed inhomogeneity of the MASP-1 catalytic region caused by deamidation as the reason behind previous unsuccessful crystallization attempts. Monitoring the separation of the various species by acidic nondenaturing PAGE during purification helped to obtain pure protein, which was subsequently crystallized.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2531277Documentos Relacionados
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