Purification and Properties of Ornithine Racemase from Clostridium sticklandii

AUTOR(ES)

Chen, Hao-Ping

FONTE

American Society for Microbiology

RESUMO

Ornithine racemase has been purified to homogeneity from Clostridium sticklandii, as shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This is the first racemase known to be highly specific to ornithine. This PLP-dependent enzyme has an Mr of 92,000, with a Km for l-ornithine of 0.77 ± 0.05 mM and a kcat of 980 ± 20 s−1.

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