Proteínas marcadoras do exercício físico aeróbio em tecido muscular de ratos treinados em diferentes intensidades. / Marker proteins of aerobic exercises in muscle tissue of rats trained at different intensities.

André Accioly Nogueira Machado

Years ago physical exercise has been used both as a tool in promoting health and in search for a better performance. Muscle tissue, which is directly involved with exercise, is a dynamic tissue, with high adaptive capacity, whose cells pass through a constant remodeling in response to different functional demands. This adaptive process is related to transcription of specific genes, unleashing structural and functional changes. Thus, this work purpose characterizes aerobic induced exercise at different intensities by the identification of proteins potential markers in skeletal muscle tissue, together with the analysis of skeletal and cardiac muscle fiber morphological patterns. Eighteen male Wistar rats were divided into three groups and submitted to an exercise training program on treadmill for 12 weeks, in different intensities. After training the animals were sacrificed for further histological analysis of skeletal and cardiac muscle and proteomic analysis of skeletal muscle. The animals of all experimental groups had a normal development in relation to body weight. The low-intensity training protocol was more effective in provoke favorable physiological adaptations, evidenced by the hypertrophy in cardiac and skeletal muscle fibers and greater cardiac vascular system. The protocol used for protein identification identified nine proteins, including enzymatic proteins such as glyceraldehyde-3-phosphate dehydrogenase, aldolase and carbonic anhydrase, and structural proteins such as actin and troponin I. Other proteins identified in our study were the myosin light chain isoforms (MLC1s, MLC1f, MLC2f and MLC3f), showing a significant variation between these different isoforms. The results suggest that lowintensity exercises have a greater ability to generate the physiological adjustments caused by aerobic training and quantitative studies could characterize the identified proteins as possible markers for the study of adaptation of skeletal muscle.

Proteínas marcadoras do exercício físico aeróbio em tecido muscular de ratos treinados em diferentes intensidades. / Marker proteins of aerobic exercises in muscle tissue of rats trained at different intensities.

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