Protein secretion in Pseudomonas aeruginosa: molecular cloning and characterization of the xcp-1 gene.

AUTOR(ES)

Bally, M

RESUMO

Pleiotropic mutations (xcp) affecting the secretion of proteins in Pseudomonas aeruginosa have been previously characterized and mapped at three different loci on the chromosome. The xcp-1 gene, which is located at the 0-min region, was isolated from a genomic bank containing DNA from P. aeruginosa PAO1. The recombinant cosmid pLX25 complemented the xcp::Tn5-751 insertion mutation previously described. The xcp-1 gene was located on pLX25 by mapping the insertion point of transposon Tn5-751 and by deletion and subcloning analysis. The xcp-1 gene was expressed when transcription was initiated from a tac promoter, and a 26,000-dalton protein was identified in Escherichia coli minicells. The Xcp-1 protein was associated with the membrane fraction of E. coli. A 30-kilodalton outer membrane protein was also affected by the xcp::Tn5-751 mutation in P. aeruginosa. The possible correlation between Xcp-1 and this protein is discussed.

Documentos Relacionados

• Outer membrane protein H1 of Pseudomonas aeruginosa: purification of the protein and cloning and nucleotide sequence of the gene.
• Protein secretion in Pseudomonas aeruginosa: the xcpA gene encodes an integral inner membrane protein homologous to Klebsiella pneumoniae secretion function protein PulO.
• Regulation of glycerol metabolism in Pseudomonas aeruginosa: characterization of the glpR repressor gene.
• Molecular characterization and nucleotide sequence of the Pseudomonas aeruginosa elastase structural gene.
• Molecular cloning and characterization of a chemotactic transducer gene in Pseudomonas aeruginosa.