Properties of triple helices formed by parallel-stranded hairpins containing 8-aminopurines
AUTOR(ES)
Aviñó, Anna
FONTE
Oxford University Press
RESUMO
Parallel-stranded hairpins with a polypyrimidine sequence linked to a complementary purine carrying 8-aminopurines such as 8-aminoadenine, 8-aminoguanine and 8-aminohypoxanthine bind polypyrimidine sequences complementary (in an antiparallel sense) to the purine part by a triple helix. The relative stabilities of triplexes were assessed by UV-absorption melting experiments as a function of pH and salt concentration. Hairpins carrying 8-aminopurines give very stable triple helical structures even at neutral pH, as confirmed by gel-shift experiments, circular dichroism and nuclear magnetic resonance spectroscopy. The modified hairpins may be redesigned to cope with small interruptions in the polypyrimidine target sequence.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=117286Documentos Relacionados
- Formation of a parallel-stranded DNA homoduplex by d(GGA) repeat oligonucleotides.
- Parallel-stranded duplex DNA containing blocks of trans purine-purine and purine-pyrimidine base pairs.
- NMR structure of a parallel-stranded DNA duplex at atomic resolution
- Structure and drug interactions of parallel-stranded DNA studied by infrared spectroscopy and fluorescence.
- Alternating d(G-A) sequences form a parallel-stranded DNA homoduplex.
