Promoter Specificity Elements in Pseudomonas aeruginosa Quorum-Sensing-Controlled Genes
AUTOR(ES)
Whiteley, Marvin
FONTE
American Society for Microbiology
RESUMO
The LasR-dependent and RhlR-dependent quorum-sensing systems are global regulators of gene expression in Pseudomonas aeruginosa. Previous studies have demonstrated that promoter elements of the quorum-sensing-controlled genes lasB and hcnABC are important in density-dependent regulation. We have identified LasR- and RhlR-dependent determinants in promoters of quorum-sensing-controlled genes qsc102, qsc117 (acpP), and qsc131 (phzA to -G) by in silico, deletion, point-mutational, and primer extension analyses. Each of these genes (in addition to lasI and rsaL) is activated by LasR, and qsc117 and qsc131 also respond to RhlR. Point mutations in the promoters of the LasR-specific gene, qsc102, relax specificity so that this promoter can respond to RhlR in addition to LasR. Our findings indicate that quorum-sensing-controlled promoters in P. aeruginosa are either specific for LasR or respond to both LasR and RhlR and that critical bases in the promoter elements determine specificity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=95443Documentos Relacionados
- Identification of genes controlled by quorum sensing in Pseudomonas aeruginosa
- Promoter specificity in Pseudomonas aeruginosa quorum sensing revealed by DNA binding of purified LasR
- Identification, Timing, and Signal Specificity of Pseudomonas aeruginosa Quorum-Controlled Genes: a Transcriptome Analysis†
- Vfr controls quorum sensing in Pseudomonas aeruginosa.
- Azithromycin Inhibits Quorum Sensing in Pseudomonas aeruginosa
