Production of native flagellin from Salmonella Typhimurium in a bioreactor and purification by tangential ultrafiltration
AUTOR(ES)
Oliveira, B. H., Silva, M. R., Braga, C. J. M., Massis, L. M., Ferreira, L. C. S., Sbrogio-Almeida, M. E., Takagi, M.
FONTE
Brazilian Journal of Chemical Engineering
DATA DE PUBLICAÇÃO
2011-12
RESUMO
Flagellin is the structural protein and most abundant component of bacterial flagella. The flagellum filament contains around 20,000 - 100,000 subunits of 50 kDa flagellin that can have diverse biotechnological applications such as vaccine adjuvant and cellular protector during chemo- and radiotherapy. The main aim of this work was to study a production process of purified native FliC flagellin of Salmonella Typhimurium. The culture conditions in shakers were established with medium devoid of animal-derived components. In bioreactors, culture conditions were established in order to obtain flagellin from the culture supernatant by tangential ultrafiltration (TUF). The concentrated 750 kDa cut-off TUF fraction had a purification factor of 1.5 and a recovery yield of 52.2% for flagellin. The volumetric production of flagellin using the described procedure achieved around 307 mg/L of culture, which represented a significant improvement over previously reported methods. These results permit the development of production and purification processes that can be easily scaled up.
Documentos Relacionados
- Excretion of flagellin by a short-flagella mutant of Salmonella typhimurium.
- In vitro polymerization of flagellin excreted by a short-flagellum Salmonella typhimurium mutant.
- Methylation of the Flagellin of Salmonella typhimurium
- Purification and characterization of a tRNA methylase from Salmonella typhimurium.
- Excretion of unassembled flagellin by Salmonella typhimurium mutants deficient in hook-associated proteins.