Preparação de colunas para cromatografia liquida de alta eficiencia recheadas com polissiloxanos (C1, C8 e C14) imobilizados sobre particulas de silica porosa de 5mum

AUTOR(ES)
DATA DE PUBLICAÇÃO

2004

RESUMO

In this work, reversed phases for High-Performance Liquid Chromatography (HPLC) were obtained by deposition of polysiloxanes with different lateral chains, C1 (polydimethylsiloxane), C8 (polymethyloctylsiloxane) and C14 (polymethyltetradecylsiloxane), in HPLC silica particles, followed by immobilizatíon using different processes: thermal treatment, self-immobilization and irradiation with microwaves and gamma radiation. Physical-chemical characterization, chromatographic evaluation and a comparative stability study of the stationary phases were carried out.The results show that different immobilization processes provide different physical characteristics in the prepared phases and that they are appropriate for chromatographic use in the separation of compounds with varied natures and polarities and also under real analysis conditions. The main advantages of these stationary phases are related to facility and cost of preparation and greater covering of the silanol groups of the silica suport that results in superior performance in the separations of basic compounds. All the stationary phases were quite stable to neutral mobile phase, with less stability at higher pH.

ASSUNTO(S)

cromatografia liquida fase estacionaria (cromatografia)

ACESSO AO ARTIGO

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