Preliminary X-ray crystallographic studies of Bacillus subtilis SpeA protein
AUTOR(ES)
Liu, Xiao-Yan
FONTE
International Union of Crystallography
RESUMO
In order to further illustrate the catalytic mechanism of arginine decarboxylase by determining the three-dimensional structure of the enzyme the speA gene was amplified from B. subtilis genomic DNA and cloned. The enzyme was expressed in Escherichia coli and purified to homogeneity by nickel-chelation chromatography followed by size-exclusion chromatography. High-quality crystals were obtained using the hanging-drop vapour-diffusion method at 298 K.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2650450Documentos Relacionados
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