Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1

AUTOR(ES)

Cândido, AL, Bontempo, ED, Resende, M

FONTE

Memórias do Instituto Oswaldo Cruz

DATA DE PUBLICAÇÃO

2000-04

RESUMO

Especial conditions were developed for the amplification of five DNA segments from US region of BHV-1 by polymerase chain reaction. In order to eliminate most nonspecific products it was found that addition of three cosolvents DMSO, glycerol and NP 40 was a simple method for increasing the specificity of amplification.

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