Point mutations in the 3' minor domain of 16S rRNA of E.coli.

AUTOR(ES)

Jemiolo, D K

RESUMO

Point mutations were produced near the 3' end of E. coli 16S rRNA by bisulfite mutagenesis in a 121 base loop-out (1385 to 1505) in a heteroduplex of wild type (pKK3535) and deletion mutant plasmids. Two highly conserved, single stranded regions flank an irregular helix (1409-1491) in the area studied. Only a single mutation was isolated in the flanking regions, a transition at C1402, (normally methylated on the base and ribose in rRNA). Mutations occurred throughout the irregular helix. All mutant rRNAs were processed and assembled into 30S subunits capable of interacting with 50S subunits. Growth rates ranged from faster to significantly slower than cells with the wild type transcript. In particular, mutations at C1467 or C1469 cause slow growth. These two transitions (in a bulge region within the helix) reduced the bulge by additional base pairing.

Documentos Relacionados

• Mutations in E.coli 16s rRNA that enhance and decrease the activity of a suppressor tRNA.
• Homology of the 3' terminal sequences of the 18S rRNA of Bombyx mori and the 16S rRNA of Escherchia coli.
• Independent in vitro assembly of a ribonucleoprotein particle containing the 3' domain of 16S rRNA.
• DNA sequence of the 3' end of the Caulobacter crescentus 16S rRNA gene.
• Binding of ribosomal protein S1 of Escherichia coli to the 3' end of 16S rRNA.