Pi, a pre-B-cell-specific enhancer element in the immunoglobulin heavy-chain enhancer.
AUTOR(ES)
Libermann, T A
RESUMO
We have identified a new immunoglobulin heavy-chain enhancer element, designated pi, between the microE2 and microE3 elements. The pi enhancer element is transcriptionally active primarily during early stages of B-cell development but becomes virtually inactive during B-cell maturation at about the stage of immunoglobulin kappa light-chain gene rearrangement. Mutational analysis suggests that the pi element is crucial for immunoglobulin heavy-chain enhancer activity at the pre-B-cell stage but is almost irrelevant for enhancer activity at the mature B-cell or plasma-cell stage. The activity of the pi enhancer element correlates with the presence of an apparently pre-B-cell-specific protein-DNA complex. The similarity of the pi site to recognition sequences for members of the ets gene family suggests that the protein(s) interacting with the pi site most likely are ets-related transcription factors.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=364640Documentos Relacionados
- Localization of a repressive sequence contributing to B-cell specificity in the immunoglobulin heavy-chain enhancer.
- Complex protein binding within the mouse immunoglobulin heavy-chain enhancer.
- Nuclear factors binding to the human immunoglobulin heavy-chain gene enhancer.
- B-lymphocyte targeting of gene expression in transgenic mice with the immunoglobulin heavy-chain enhancer.
- Negative regulation contributes to tissue specificity of the immunoglobulin heavy-chain enhancer.
