Physicochemical characteristics and antioxidant activity of melanoidin pigment from the fermented leaves of Orthosiphon stamineus

AUTOR(ES)

Olennikov, Daniil N., Tankhaeva, Larisa M.

FONTE

Revista Brasileira de Farmacognosia

DATA DE PUBLICAÇÃO

21/10/2011

RESUMO

The melanoidin pigment (OS-M) was isolated from fermented leaves of Orthosiphon stamineus Benth. (Lamiaceae), with a 0.37% yield (from dry plant weight), and characterised. OS-M is a phenolic polymer with a molecular weight Keywords: of 4.4 kDa. Determination of basic physicochemical parameters using elemental antioxidant activity analysis, functional group analysis, UV-Vis-and FTIR-spectroscopy of OS-M fermented leaves indicated that the isolated polymer was similar to typical melanoidins. Experimental Lamiaceae data show that aromatic fragments dominate the OS-M structure, which also contains melanoidin a small amount of aliphatic fragments. Investigation into the antioxidant activity of Orthosiphon stamineus Benth OS-M under in vitro conditions demonstrated that O. stamineus melanoidin has a scavenging effect against free radicals (DPPH•, ABTS•+, O2•-) and NO molecules, inactivates molecules of H2O2, chelates Fe2+-ions and oxidises NADH.

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