Physical consequences of chemical modification: circular dichroism of the kethoxalated oligoribonucleotides

AUTOR(ES)

Poletayev, A.I.

RESUMO

Alterations in CD spectra are found in G-containing oligoribonucleotides after modification with kethoxal (β-ethoxy--α-ketobutyraldehyde). Stacking interactions in kethoxalated oligomers are followed by temperature dependence of their CD amplitudes. It is shown that for oligomers with nucleosides in anti-conformation adduct formation destroys the stacking interaction with 3′-neighbour but not with a 5′-neighbour. For nucleosides in non-standard conformation (i.e. syn-conformation of guanine in GpGpCp) the physical alteractions may be seen in those cases, when the substituting group affects the initial conformation or the interplane base contacts via, for instance, blocking NH2-group of guanine in GpUp.

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