Physical characterization of the ilvHI operon of Escherichia coli K-12.

AUTOR(ES)

Squires, C H

RESUMO

The ilvHI and leu genes of Escherichia coli K-12 are contained on a single 10.9-kilobase EcoRI fragment of deoxyribonucleic acid derived from the leu transducing phage lambda G4. Since the expression of all of these genes is controlled by leucine, we investigated whether they are part of single operon or whether they constitute separate but adjacent operons controlled from a common site. Both cloning and hybridization studies indicated that ilvHI and leu are distinct operons. They are transcribed in opposite directions and are separated by approximately 1,500 base pairs of deoxyribonucleic acid. Hybridization experiments showed that the expression of ilvHI is regulated chiefly at the level of transcription. The size of the ilvHI messenger ribonucleic acid is estimated to be 2,550 bases.

Documentos Relacionados

• Physical analysis of deletion mutations in the ilvGEDA operon of Escherichia coli K-12.
• The complete nucleotide sequence of the ilvGMEDA operon of Escherichia coli K-12.
• Specificity of attenuation control in the ilvGMEDA operon of Escherichia coli K-12.
• IlvHI locus of Salmonella typhimurium.
• Point mutations in the regulatory region of the ilvGMEDA operon of Escherichia coli K-12.