Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
AUTOR(ES)
Vaucher, Rodrigo de Almeida, Dezen, Diogenes, Simonetti, Amauri Braga, Spilki, Fernando Rosado, Roehe, Paulo Michel
FONTE
Braz. J. Microbiol.
DATA DE PUBLICAÇÃO
2011-12
RESUMO
Genomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phylogenetic comparison with reference strains of bPI3V. The Brazilian isolates revealed a high degree of genomic when compared to SF4/32 prototype strain, within the recently proposed genotype A of bPIV-3.
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