Photodynamic therapy effect in carious bovine dentin : an in vitrostudy / Estudo da ação antimicrobiana da terapia fotodinamica sobre lesões de carie produzidas in vitro na dentina de dentes bovinos

AUTOR(ES)

Marcela Pinto Monteiro de Oliveira

DATA DE PUBLICAÇÃO

2010

RESUMO

Photodynamic therapy (PDT) is a technique that consists in the activation of certain photosensitizers by light in the presence of tissue oxygen, resulting in the production of reactive radicals capable of inducing cell death. In this context, this therapy may become a suitable approach to disinfect the dentin tissue during the caries treatment, and reduce the tissue removal, minimizing the probability of caries progression and pulp involvement. This randomized in vitro study determined parameters for using a light-emitting diode (LED) with toluidine blue O (TBO) for reduction of Streptococcus mutans counts inside dentin caries. Seventy two bovine coronary dentin slabs were immersed in Streptococcus mutans culture for demineralization production. Dentin slabs were allocated to 6 groups (n=12) as follows: Control (treated with 0.9% NaCl solution for 5 min); TBO (treated with 0.1 mg/ml TBO for 5 min); LEDA (submitted to irradiation for 4.2 min); LEDB (submitted to irradiation for 6.5 min); PDTA (treated with TBO plus irradiation for 4.2 min) and PDTB (treated with TBO plus irradiation for 6.5 min). The energy densities used for 4.2 and 6.5 min correspond at 166 and 249 J/cm2, respectively. Before and after treatments, dentin samples were analyzed with regard to S. mutans counts. The caries lesion depth produced by the microbiological model was analyzed by polarized light microscopy. ANOVA/Tukey tests were utilized to compare log reductions among groups (α=5%). Bacterial reduction was observed when dentin was exposed to both TBO and LED at both irradiation times. However, no difference in S. mutans reduction was found between the two energy densities. Concluding, although the use of LED combined with TBO was effective in reducing the Streptococcus mutans counts in carious dentin, this effect may not have clinical significance.

ASSUNTO(S)

streptococcus mutans streptococcus mutans

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