Phosphoenolpyruvate Carboxykinase Activity in Grape Berries 1

AUTOR(ES)

Ruffner, H. P.

RESUMO

Phosphoenolpyruvate (PEP) carboxykinase activity was found in crude extracts of `Pinot noir' grape berries. The enzyme required ATP, Mn2+ plus Mg2+, a pH of 6.6, and a temperature of 40 C for maximum activity. The range in concentration of oxaloacetic acid needed for maximum phosphoenolpyruvate carboxykinase activity was 5 to 10 mm, and the Km for HCO3- in the exchange of 14CO2 into oxaloacetic acid was 26.8 mm.

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