Perfil genotípico de cepas de Listeria monocytogenes isoladas de alimentos: análise crítica das técnicas de PCR e PFGE e importância para a Saúde Pública. / Genotype profile of cepas of would listeria monocytogenes isolated of foods: critical analysis of the techniques of PCR and PFGE and importance for the public health.
AUTOR(ES)
Gillian Alonso Arruda
DATA DE PUBLICAÇÃO
2006
RESUMO
Due to the seriousness of clinical manifestations and high rates of mortality of the listeriosis disease in populations at risk, control and prevention of this disease, caused by L. monocytogenes, represent an important challenge to sanitation authorities. Difficulties of recovering organisms involved in outbreaks; sub notification; and, the delay in accomplishing conventional analysis are some of the factors that slow-down or even prevent health interventions in outbreak occurrences. With the advent of the molecular biology, new techniques have been employed for identifying and typifying the L. monocytogenes aiming at contributing to studies on epidemiology and sanitary surveillance of foods. The present study aimed at ratifying the taxonomy and the genotypic profile of 31 isolated stocks of varied types of meats supposedly identified as L. monocytogenes by means of the Polymerase Chain Reaction (PCR) employing identification starters of genes 16 rRNA and A and B Internalin in order to confirm the species. Results indicated that only 20 (65.0%) of the stocks were confirmed as being from the investigated species. Another evaluation of similarity were also carried out along with the 20 stocks ratified through pulsed field gel electrophoresis (PFGE), which disclosed a great genotypic diversity characterized by 18 different profiles, according to the Pearsons similarity coefficient. Results demonstrated that the molecular techniques are very helpful for identifying and typifying the L. monocytogenes, characterizing themselves as important epidemiological tools.
ASSUNTO(S)
listeria monocytogenes vigilância pfge sanitary surveillance internalina 16s 16s internalin pcr pfge listeria monocytogenes pcr
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