Perfil bioquímico e proteômico do recém nascido pequeno para a idade gestacional: pesquisa de biomarcadores para hipertensão arterial na vida adulta. / Biochemical and proteomic profile of newborns small for gestational age: search for biomarkers for hypertension in adulthood.

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

15/03/2011

RESUMO

Introduction: systemic arterial hypertension is a serious public health problem. A strong correlation between birthweight and mortality and morbidity from hypertension and cardiovascular disease formed the basis for the fetal programming hypothesis for the non communicable diseases of adulthood; also known as Barkers hypothesis of hypothesis of the developmental origins of health and disease. Intrauterine growth is a complex phenomenon, which results from the interaction of countless factors. Customized criteria for its classification have been proposed but not incorporated, or even validated, in clinical practice in most neonatology centres in our Country. Objective: to identify biomarkers for fetal programming in small for gestational age babies as a preventive strategy to systemic hypertension in adulthood. Specifically, we evaluated biochemical markers in SGA babies classified by both classical and customized both criteria and studied their proteome in maternal and umbilical cord blood serum. Methods: Thirty-two pregnant women and their fetuses were evaluated. They were selected as small for gestational age (st-SGA=8) or adequate for gestational age (st-AGA=24) by standard criteria. Serum levels of nitric oxide, high sensitive C reactive protein (hs-CRP), uric acid, lipids and proteins were assessed in both maternal and umbilical cord blood. Neonatal proteome was also analysed through trypsin treatment of selected proteins, MALDI-TOF analysis and identification in the Swiss Prot database. Results: The application of customized growth criteria yield three new groups: 16 adequate for gestational age (ct-AGA), 13 small for gestational age (ct-PIG) and three large for gestational age (3-GIG) babies. Biochemical analysis showed higher nitric oxide and hs-CRP levels in both st-SGA and ct-SGA. Mothers of these babies had lower albumin levels. St-SGA babies had higher triglyceride and cholesterol levels. Ct-LGA babies and mothers had higher uric acid levels, and these mothers had also higher triglycerides. Proteomic analysis showed differences in protein expression between AGA and SGA babies. Proteins minimally expressed in SGA, and one other was over expressed in SGA babies were Kruppel-like factor 10 (KLF10), low molecular weight phosphotyrosine protein phosphatase (PPAC), epididymal-specific lipocalin (LCN8), gastrin-releasing peptide (GRP), interferon epsilon (IFNE), and BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 1 (BACD1). The protein over expressed in SGA babies was a WAP four-disulfide core domain protein 8 (WFDC8). Conclusions: Small for gestational age babies show different biochemical and proteomic expression in relation to adequate for gestational age babies. Customized growth criteria seem more accurate to identify these individuals. These findings can be helpful to the development of biochemical and proteomic markers for babies at risk for adverse fetal programming.

ASSUNTO(S)

programação fetal medicina hypertension intrauterine growth restricted fetal programming hipertensão arterial sistêmica crescimento intra-uterino restrito

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