Partial VP2 sequencing of canine parvovirus (CPV) strains circulating in the state of Rio de Janeiro, Brazil: detection of the new variant CPV-2c
AUTOR(ES)
Castro, T.X., Costa, E.M, Leite, J.P.G., Labarthe, N.V., Cubel Garcia, R.C.N.
FONTE
Brazilian Journal of Microbiology
DATA DE PUBLICAÇÃO
2010-12
RESUMO
Canine parvovirus (CPV) is the most important enteric virus for dogs and it seems to be undergoing continuous evolution, generating new genetic and antigenic variants throughout the world. The aim of this study was to analyze the distribution of CPV variants from 1995 to 2009 and to investigate the circulation of the new variant CPV-2c in Rio de Janeiro, Brazil. In addition, the clinical features of CPV infection were also reported. After CPV laboratorial confirmation by HA/HI and PCR, thirty-two fecal samples were analyzed by sequencing a 583-bp fragment of the VP2 gene. One sample, collected in 2008 was typed as the new type CPV-2c. All samples from 1995 to 2003 were identified as "new CPV-2a". From 2004 to 2006, both "new CPV-2a" and CPV-2b were observed. From 2006 to 2009, most of the samples were characterized as CPV-2b. The classical signs of CPV enteritis were observed in 16/18 CPV-2a and 5/13 CPV-2b infected puppies. These results show that continuous epidemiological surveillance of CPV strain distribution is essential for studying the patterns of CPV-2a and 2b spread and for determining whether the new variant CPV-2c has become permanently established in Brazilian canine population.
Documentos Relacionados
- Canine Parvovirus (CPV) Vaccination: Comparison of Neutralizing Antibody Responses in Pups after Inoculation with CPV2 or CPV2b Modified Live Virus Vaccine
- First detection of canine parvovirus type 2c in Brazil.
- Identification of domains in canine parvovirus VP2 essential for the assembly of virus-like particles.
- Aspectos clínicos e epidemiológicos da enterite por parvovírus canino (CPV) no estado do Rio de Janeiro: 1995-2004
- Isolation and characterization of canine parvovirus type 2c (CPV-2c) from symptomatic puppies