Padrão de expressão gênica e localização tecidual no rato de um novo membro do Cluster gênico da enzima conversora da angiotensina I: variante-4 / Gene and tissue expression pattern of a novel member of the angiotensin converting enzyme-I gene cluster in the rat: variant-4

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

The renin-angiotensin system (RAS) is essential to maintain the cardiovascular homeostasis. The angiotensin-converting enzyme (ACE) is a critical point in the biochemical activation of several active substances, notably angiotensin II. Evidence obtained in our laboratory using comparative genomic analysis and confirmed by cDNA cloning suggests that this protein family is incomplete and point to the existence of two new isoforms of ACE that from now on are denominated Variant-3 (Var-3) and Variant-4 (Var-4), located within the same ACE locus. In the present work we simultaneously analyzed the expression pattern of the 4 ACE gene variants in 30 different tissues of rats. The variant 4, whose mechanism of action remains unknown and it is being presently investigated in our laboratory is mainly expressed in testis and in relatively low quantity in left ventricle. Using in situ hybridization technique in testis, we verified that positive labeling of Var-4 is distinct from Var-2, suggesting that they may play distinct functions during the spermatogenesis process. Taking together, we provide direct evidence that the ACE gene locus contain, 4 variants instead of 2 and they show a specific cell tissue pattern of expression. Mostly important, the Var-4 is primarily expressed in testis and the data suggest that it may be involved with spermatogenesis control, and in cardiac processes presently unknown

ASSUNTO(S)

expressão gênica in situ hibridization hibridização in situ pepdil dipeptidase a peptídil dipeptidase a sistema renina-angiotensina wistar rats polimerase chain reaction (pcr) renin-angiotensin system gene expression ratos wistar reação em cadeia da polimerase

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