Optimization of Procedures for Isolation of Mycobacteria from Soil and Water Samples Obtained in Northern India

AUTOR(ES)

Parashar, Deepti

FONTE

American Society for Microbiology

RESUMO

For isolation of environmental mycobacteria, a decontamination procedure has been standardized by which treatment with 3% sodium dodecyl sulfate plus 4% NaOH (15 and 30 min for rapid and slow growers, respectively) is followed by incubation with 2% cetrimide (5 and 15 min for fast- and slow-growing mycobacteria, respectively); this procedure was found to completely eliminate contamination with other organisms and resulted in the isolation of only mycobacteria.

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