One-Step PCR To Distinguish B Virus from Related Primate Alphaherpesviruses
AUTOR(ES)
Hirano, Makoto
FONTE
American Society for Microbiology
RESUMO
By adding betaine to the PCR mixture, we previously established a PCR method to amplify a DNA segment of the glycoprotein G gene of B virus (BV) derived from a rhesus macaque. We have found that DNA of other BV strains derived from cynomolgus, pigtail, and lion-tailed macaques can also serve as the template in our PCR assay. Under the same conditions no product was obtained with DNA of simian agent 8 of green monkeys and Herpesvirus papio 2 of baboons, or the human herpes simplex viruses types 1 and 2. Thus, this PCR method is useful to discriminate BV from other closely related primate alphaherpesviruses.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=119990Documentos Relacionados
- Directed termination PCR: a one-step approach to mutation detection.
- Inability of One-Step Real-Time PCR To Detect Borrelia burgdorferi DNA in Urine
- Detecção do vírus da cinomose canina por diferentes métodos de One-Step RT-qPCR
- Adenovirus Susceptibility to Human Interferon During One-Step Replication 1
- One-step affinity purification protocol for human telomerase.
