Oestrogen receptor analysis: correlation between enzyme immunoassay and immunohistochemical methods.
AUTOR(ES)
Aasmundstad, T. A.
RESUMO
AIMS: To compare oestrogen receptor measurements on fresh tissue done by an enzyme immunoassay (ER-EIA, Abbott) and an immunohistochemical technique (ER-ICA, Abbott) using formalin fixed, paraffin was embedded material. METHODS: The ER-EIA is based on a sandwich immunoassay using fresh frozen tissue, and the absorbance values were read by a Quantum Analyzer. Sections were cut from the corresponding paraffin wax blocks, and after pretreatment with pronase and DNase, the slides were incubated with monoclonal oestrogen antibody. The immunoperoxidase staining was scored semiquantitatively, incorporating both the intensity and percentage of positive staining (HSCORE). RESULTS: HSCORE rating of the ER-ICA slides gave a significant correlation with the quantitative ER-EIA values (r = 0.76; p less than 0.001). The overall sensitivity and specificity of the immunohistochemical method was 88% and 93%, respectively. CONCLUSIONS: ER-ICA on routinely processed material can be a valuable alternative when biochemical analysis of oestrogen receptor content is not available. Furthermore, immunohistochemical staining identifies oestrogen receptor positivity at a cellular level, which ensures that the analysed material is representative. This technique could therefore be particularly valuable in small tumours and in situ lesions.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=495650Documentos Relacionados
- Comparison of new immunohistochemical assay for oestrogen receptor in paraffin wax embedded breast carcinoma tissue with quantitative enzyme immunoassay.
- Statistical analysis of comparison between laboratory methods.
- Immunohistochemical and biochemical analysis of the oestrogen regulated protein pS2, and its relation with oestrogen receptor and progesterone receptor in breast cancer.
- Immunohistochemical quantitation of oestrogen receptors and proliferative activity in oestrogen receptor positive breast cancer.
- Assessment of inflammation in the rheumatoid knee joint: correlation between clinical, radioisotopic, and thermographic methods.