Occurrence of blaOXA-23 gene in imipenem-susceptible Acinetobacter baumannii
AUTOR(ES)
Carvalho, Karyne Rangel, Carvalho-Assef, Ana Paula D'Alincourt, Santos, Lia Galvão dos, Pereira, Maria José Félix, Asensi, Marise Dutra
FONTE
Memórias do Instituto Oswaldo Cruz
DATA DE PUBLICAÇÃO
2011-06
RESUMO
The aim of the current study was to describe the occurrence of the blaOXA-23 gene and the ISAba1 element in imipenem-susceptible Acinetobacter baumannii strains. By performing the polymerase chain reaction mapping using combinations of ISAba1 forward primers and the blaOXA-23-like gene reverse primers, we demonstrated that the ISAba1 element did not occur upstream of the blaOXA-23 gene in five of 31 isolates, which explained the lack of resistance to imipenem despite the presence of the blaOXA-23 gene. All of the blaOXA-23-positive isolates were susceptible to imipenem and meropenem with minimal inhibitory concentration < 4 µg/mL. Pulsed-field gel electrophoresis analysis revealed four genotypes among the five blaOXA-23-positive isolates. The current report of the blaOXA-23 gene in imipenem-susceptible isolates provided evidence that this gene may be silently spread in a hospital environment and highlighted the threat of undetected reservoirs of carbapenemase genes.
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