Nucleotide sequence of an insertion sequence (IS) element identified in the T-DNA region of a spontaneous variant of the Ti-plasmid pTiT37.

AUTOR(ES)

Vanderleyden, J

RESUMO

We have identified and determined the nucleotide sequence of an IS element (IS136) of Agrobacterium tumefaciens. This is the first IS element isolated and sequenced from a nopaline type Ti-plasmid. Our IS element has 32/30 bp inverted repeats with 6 mismatches, is 1,313 bp long and generates 9 bp direct repeats upon integration. IS136 has 3 main open reading frames (ORF's). Only ORF1 (159 codons) is preceded by sequences that are proposed to serve functional roles in transcriptional and translational initiation. No DNA sequence homology was found between IS136 and IS66, an IS element isolated from an octopine type Ti-plasmid.

Documentos Relacionados

• The right border region of pTiT37 T-DNA is intrinsically more active than the left border region in promoting T-DNA transformation.
• Nucleotide sequence of the insertion sequence found in the T-DNA region of mutant Ti plasmid pTiA66 and distribution of its homologues in octopine Ti plasmid.
• Nucleotide sequence of the tmr locus of Agrobacterium tumefaciens pTi T37 T-DNA.
• On the isolation of TI-plasmid from Agrobacterium tumefaciens.
• Promoters of Agrobacterium tumefaciens Ti-plasmid virulence genes.