Noncoding regulatory sequences of Ciona exhibit strong correspondence between evolutionary constraint and functional importance
AUTOR(ES)
Johnson, David S.
FONTE
Cold Spring Harbor Laboratory Press
RESUMO
We show that sequence comparisons at different levels of resolution can efficiently guide functional analyses of regulatory regions in the ascidians Ciona savignyi and Ciona intestinalis. Sequence alignments of several tissue-specific genes guided discovery of minimal regulatory regions that are active in whole-embryo reporter assays. Using the Troponin I (TnI) locus as a case study, we show that more refined local sequence analyses can then be used to reveal functional substructure within a regulatory region. A high-resolution saturation mutagenesis in conjunction with comparative sequence analyses defined essential sequence elements within the TnI regulatory region. Finally, we found a significant, quantitative relationship between function and sequence divergence of noncoding functional elements. This work demonstrates the power of comparative sequence analysis between the two Ciona species for guiding gene regulatory experiments.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=534669Documentos Relacionados
- Strong evolutionary conservation of neuropeptide Y: sequences of chicken, goldfish, and Torpedo marmorata DNA clones.
- Molecular, functional, and evolutionary analysis of sequences specific to Salmonella.
- An evolutionary constraint: Strongly disfavored class of change in DNA sequence during divergence of cis-regulatory modules
- Discovering regulatory elements in non-coding sequences by analysis of spaced dyads
- Sequences of bovine papillomavirus type 1 DNA--functional and evolutionary implications.