Nicotinamide Adenine Dinucleotide-dependent Proline Dehydrogenase in Chlorella

AUTOR(ES)

McNamer, Alan D.

RESUMO

An NAD-linked dehydrogenase from Chlorella pyrenoidosa Chick catalyzing the conversion of l-proline to Δ1-pyrroline-5-carboxylic acid was partially purified. Δ1-Pyrroline-5-carboxylic acid was identified as the product by co-chromatography of it and its o-aminobenzaldehyde derivative with authentic compounds. The enzyme is NAD and l-proline specific and is not an oxidase; NADP is not inhibitory. The Michaelis constant for NAD is 0.08 mm and for proline is 0.73 mm.

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