New region of the simian virus 40 genome required for efficient viral transformation.
AUTOR(ES)
Bouck, N
RESUMO
Viable mutants of simian virus 40 with deletions in three regions of the virus genome (map coordinates 0.21-0.17, 0.59-0.54, and 0.67-0.74) have been tested for their ability to transform rat fibroblasts to anchorage independence. Only those mutants whose deletions occur between 0.59 and 0.55 in the proximal part of the early region are defective in transforming ability. The most severely defective of these transform with less than one-hundredth the efficiency of wild type. They retain their defect when tested in Chinese hamster lung cells and when infection is initiated with viral DNA instead of intact virions. Complementation for transformation can be observed between these transformation-defective deletions and a simian virus 40 temperature-sensitive A mutant.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=392576Documentos Relacionados
- Identification of a region of simian virus 40 large T antigen required for cell transformation.
- Simian virus 40 functions required for the establishment and maintenance of malignant transformation.
- Less than 40% of the simian virus 40 large T-antigen-coding sequence is required for transformation.
- Hybrid genomes of the polyomaviruses JC virus, BK virus, and simian virus 40: identification of sequences important for efficient transformation.
- Bidirectional promoter elements of simian virus 40 are required for efficient replication of the viral DNA.