New Pathway for Long-Chain n-Alkane Synthesis via 1-Alcohol in Vibrio furnissii M1

AUTOR(ES)

Park, Myong-Ok

FONTE

American Society for Microbiology

RESUMO

Alkane biosynthesis in the bacterium Vibrio furnissii M1 involves the synthesis of long-chain alkanes via 1-alcohol. Evidence for this novel pathway are the following. (i) Both even- and odd-carbon-number n-alkanes were produced from glucose, while only even-carbon-number fatty acids were produced in V. furnissii M1. This result cannot be explained by the decarbonylation pathway. (ii) Pentadecane and hexadecane were produced from 1-hexadecanoic acid by membrane fractions of V. furnissii M1, and radioisotope precursor-tracer experiments, in which 1-[1-14C]hexadecanoic acid was fed, identified the corresponding alcohol, aldehyde, and alkane derivatives. Since all metabolites maintained the radioisotope label at 1-C, they were produced by a pathway in which the carbon structure was retained, i.e., a reduction pathway. (iii) n-Hexadecane was produced when 1-hexadecanol was fed to membrane preparations.

Documentos Relacionados

• Long-Chain Aldehyde Dehydrogenase That Participates in n-Alkane Utilization and Wax Ester Synthesis in Acinetobacter sp. Strain M-1
• Pathway of n-Alkane Oxidation in Cladosporium resinae
• N-Alkane oxidation enzymes of a pseudomonad.
• n-Alkane Utilization and Lipid Formation by a Nocardia
• Rapid synthesis of long-chain deoxyribooligonucleotides by the N-methylimidazolide phosphotriester method.