New host record of five Flavobacterium species associated with tropical fresh water farmed fishes from North India
AUTOR(ES)
Verma, Dev Kumar, Rathore, Gaurav
FONTE
Braz. J. Microbiol.
DATA DE PUBLICAÇÃO
2015-12
RESUMO
Abstract Yellow pigmented, filamentous, Gram-negative bacteria belonging to genus Flavobacterium are commonly associated with infections in stressed fish. In this study, inter-species diversity of Flavobacterium was studied in apparently healthy freshwater farmed fishes. For this, ninety one yellow pigmented bacteria were isolated from skin and gill samples (n = 38) of three farmed fish species i.e. Labeo rohita, Catla catla and Cyprinus carpio. Among them, only twelve bacterial isolates (13.18%) were identified as Flavobacterium spp. on the basis of morphological, biochemical tests, partial 16S rDNA gene sequencing and phylogenetic analysis. On the basis of 16S rDNA gene sequencing, all the 12 isolates were 97.6-100% similar to six different formally described species of genus Flavobacterium. The 16S rDNA based phylogenetic analysis grouped these strains into six different clades. Of the 12 isolates, six strains (Fl9S1-6) grouped with F. suncheonense, two strains (Fl6I2, Fl6I3) with F. indicum and the rest four strains (Fl1A1, Fl2G1, Fl3H1 and Fl10T1) clustered with F. aquaticum, F. granuli, F. hercynium and F. terrae, respectively. None of these species except, F. hercynium were previously reported from fish. All the isolated Flavobacterium species possessed the ability of adhesion and biofilm formation to colonize the external surface of healthy fish. The present study is the first record of tropical freshwater farmed fishes as hosts to five environmentally associated species of the Flavobacterium.
Documentos Relacionados
- New record of Machaeriobia machaerii (Kieffer, 1913) (Diptera, Cecidomyiidae) in Brazil and association with host-plant species
- Six new species of Poeciloderrhis from Brazil (Blattaria: Blaberidae) with a new record and a new combination
- Utilization of Low Concentrations of Starch by a Flavobacterium Species Isolated from Tap Water
- Megalorhipida leucodactyla (Fabricius) (Lepidoptera: Pterophoridae): first distribution record from Chile and new host plant record
- Chromosome evolution in fishes: a new challenging proposal from Neotropical species