Mutations Affecting the Structure of Hypoxanthine: Guanine Phosphoribosyltransferase in Cultured Chinese Hamster Cells

AUTOR(ES)

Beaudet, A. L.

RESUMO

14 Clones resistant to 8-azaguanine, isolated from mutagenically treated cultured cells from Chinese hamsters, were tested for loss of enzymic and immunological activities of hypoxanthine: guanine phosphoribosyltransferase. Three of the clones had no enzymic activity but reacted strongly with antiserum prepared against the enzyme (CRM+), indicating the presence of a defective enzyme protein, probably caused by a mutation in its structural gene. The other 11 mutants had little or no enzymic or immunologic activity. Revertant clones with positive enzymic and crossreacting immunologic activities were derived from both classes of mutants. The antiserum has been used for rapid purification of the phosphoribosyltransferase from Chinese hamster cells by immunoadsorbant affinity chromatography.

Documentos Relacionados

• Adenovirus-induced mutations at the hypoxanthine phosphoribosyltransferase locus of Chinese hamster cells.
• Hypoxanthine-guanine phosphoribosyltransferase genes of mouse and Chinese hamster: construction and sequence analysis of cDNA recombinants.
• Mutations which alter splicing in the human hypoxanthine-guanine phosphoribosyltransferase gene.
• THE PURINE REQUIREMENT OF CELLS CULTURED FROM HUMANS AFFECTED WITH THE LESCH-NYHAN SYNDROME (HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE DEFICIENCY)*
• 8-Azaguanine Resistance in Mammalian Cells I. Hypoxanthine-Guanine Phosphoribosyltransferase