Mutant of Yeast Sensitive to 2,6-Diaminopurine
AUTOR(ES)
Lomax, Christopher A.
RESUMO
A mutant of yeast sensitive to growth inhibition by 2,6-diaminopurine (2,6-DAP) was analyzed genetically and found to be a double mutant. One gene, dap, conferred approximately 30% sensitivity to the analogue. The other, slw, potentiated the inhibition such that the double mutant dap slw was inhibited 90%. The mutation dap conferred concomitant sensitivity to a number of other purine analogues. The activity of a purine phosphoribosyltransferase with 2,6-DAP in a strain carrying dap was found to be three times higher than in the wild type. It is inferred that the mutation alters the properties of a purine phosphoribosyltransferase. A possible mechanism for the effect of slw is also discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=250163Documentos Relacionados
- Utilization of 2,6-diaminopurine by Salmonella typhimurium.
- Adenine and 2,6-diaminopurine as germinants for Bacillus macerans spores.
- Thermodynamic studies of base pairing involving 2,6-diaminopurine.
- Increased DNA binding and sequence discrimination of PNA oligomers containing 2,6-diaminopurine.
- Comparative mutagenicities of N6-methoxy-2,6-diaminopurine and N6-methoxyaminopurine 2'-deoxyribonucleosides and their 5'-triphosphates.